RARalpha Human Retinoic Acid NHR Binding Agonist Radioligand LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Retinoic acid receptors (RARs) are retinoic acid inducible enhancer factors belonging to the superfamily of steroid/thyroid receptors. The retinoic acid metabolite has profound effects on vertebrate development. Retinoic acid is a morphogen and a powerful tetragen. This receptor controls cell function by directly regulating gene expression. Inhibition of RARA (RAR α, NR1B1) may be useful in the treatment of various cancers.
Family:
NHR
Sub Family:
Retinoic Acid
Class:
Non-Steroid
Protein Name:
RARalpha
Uniprot Number:
P10276
Protein Aliases:
Nuclear receptor subfamily 1 group B member 1
Gene Name:
RARA
Gene ID:
5914
Gene Aliases:
RAR|NR1B1
Accession Number:
XM_017024920
Organism:
Human
Construct Details:
Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Binding
Detection Method:
Radiometric
Measured Response:
Scintillation
Testing Information
Ligand:
[3H]9-cis retinoic acid
Ligand Kd (nM):
0.52
Ligand Concentration:
1 nM
Non Specific:
AM580 (1 µM)
Incubation:
120 min at 4°C
Control Inhibitor:
AM580
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 μl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Adverse / Beneficial Action:
Inhibition of RARA (RAR , NR1B1) may be useful in the treatment of various cancers.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
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