CHT1 Human Choline Transporter Binding Antagonist Radioligand LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Choline transport is the rate limiting step for acetylcholine production. A high-affinity sodium-dependent uptake mechanism in synaptosomes plays a critical role in the regulation of acetylcholine synthesis in cholinergic neurones. Inhibition of choline transport and uptake may cause nerve depletion of acetylcholine resulting in decreased cholinergic function; i.e., loss of memory and gastrointestinal function, tachycardia, etc.
Family:
Transporter
Sub Family:
SLC5 / Choline
Class:
SLC
Protein Name:
CHT1
Uniprot Number:
Q9GZV3
Gene Name:
SLC5A7
Gene ID:
60482
Gene Aliases:
hCHT|CHT1
Accession Number:
XM_017004628
Organism:
Human
Construct Details:
Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Binding
Detection Method:
Radiometric
Measured Response:
Scintillation
Testing Information
Ligand:
[3H]hemicholinium-3
Ligand Kd (nM):
3.9
Ligand Concentration:
3 nM
Non Specific:
10 µM Hemicholinium-3
Incubation:
60 min at RT
Control Inhibitor:
hemicholinium-3
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 μl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
2
Turnaround Time
Standard:
10 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
CNS/Addiction/Pain
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
BioPrint SafetyScreen Panel - FR
Item: P22-p
Diversity LeadHunter Panel - FR
Item: P9
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