Biological Information

Family:

Transporter

Class:

ATPase

Protein Name:

Na/K ATPase

Uniprot Number:

P05023

Gene Name:

ATP1A1

Gene ID:

476

Organism:

Pig

Tissue:

Brain

Assay Information

Assay Type:

Biochemical

Assay Sub Type:

Enzymatic

Functional Mode:

Antagonist

Detection Method:

Spectrophotometric

Testing Information

Substrate:

ATP (2 mM)

Incubation:

60 min at 37°C

Control Inhibitor:

ouabain

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.

Test Sample Requirements:

Minimum for 1) Screen: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 μl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.

Minimum Order Quantity:

2

Turnaround Time

Standard:

15 Business Days

Reference Compound Data

PubChem ID

439501

Name

Ouabain

Measurement

249nM - IC50

PubChem ID

92132052

Name

melittin

Measurement

- IC50

PubChem ID

Name

buffalin

Measurement

190nM - IC50

Clinical Relevance

Therapeutic Area:

CNS/Addiction/Pain

Additional Information

Brand:

LeadHunter

Testing Location:

France - Celle L'Evescault

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