5-HT1D Rat Serotonin GPCR Binding Agonist Radioligand LeadHunter Assay - FR

Ligand:

[3H]serotonin

Ligand Kd (nM):

0.5

Ligand Concentration:

1 nM

Non Specific:

10 µM Serotonin

Incubation:

60 min at RT

Control Inhibitor:

serotonin

Test Concentration / Dose:

Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.

Test Sample Requirements:

Minimum for 1) Screen: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 μl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.

Minimum Order Quantity:

2