Immunophenotyping identifies cells based on proteins expressed on the cell surface. For therapies with intentional, unintentional, and even undetermined impact on distribution of immune cell subpopulations, we offer flow cytometry assessment of test molecules and reference or standard-of-care therapeutics on peripheral blood mononuclear cells (PBMCs).
Figure 1. Flow cytometry of human PBMCs with eight color analysis identifies subpopulations of B cells, T cells, and monocytes.
Immune cell profiling for test agent-induced changes in biomarkers that specifically identify T cells, B cells, NK cells, and monocyte subpopulations, within pooled or single-donor PBMCs, may predict subsequent cytokine and chemokine secretion responses in patients. This, in turn, may influence intended target modulation and thus is an important component of both small and large molecule discovery programs. With our immunophenotyping services, you can:
- Determine immune population ratios and activation status
- Identify potential shifts in cytokine and chemokine secretion resulting from test agent treatment
- Identify potential modulation of antibody production by test agent treatment
- Gain insights on immunomodulatory efficacy, safety, and test agent mechanism-of-action
Obtain a more comprehensive understanding of the complexity of the human immune response with data on B cells, T cells, and monocyte populations.
