Yoph Yersinia Phosphatase Enzymatical LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
YopH is a Yersinia protein that has protein tyrosine phosphate phosphatase activity (PTPase). YopH is required for virulence and has homology to eukaryotic SH2 proteins and SH3 binding domains. YopH breaks down signal transduction mechanisms in immune cells and inhibits the immune response. It contributes to the ability of the bacteria to resist phagocytosis by peritoneal macrophages.
Family:
Phosphatase
Sub Family:
Non-Receptor
Class:
Protein Tyrosine
Protein Name:
YopH
Uniprot Number:
P15273
Gene Name:
yopH
Gene ID:
N/A
Gene Aliases:
yop15
Accession Number:
NC_002120
Organism:
Bacteria
Construct Details:
Full length; R211A, Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Recombinant protein phosphatase YopH expressed in E. coli cells is used. Test compound and/or vehicle is preincubated with 0.013 μg/ml enzymeõ for 15 minutes at 37°C in modified HEPES buffer pH 7.2. The reaction is initiated by addition of 20 μM DiFMUP for 30 minutes. Determination of the amount of DiFMU formed is read spectrofluorimetrically at 358 nm/450 nm. Compounds are screened at 10 μM.
Substrate:
20 µM DiFMUP
Incubation:
30 min at 37°C
Control Inhibitor:
(NH₄)₆Mo₇O₂₄
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Gastroenterology
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
RELATED SOLUTIONS
Phosphatase Profiler LeadHunter Panel - TW
Item: PP260
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