Biological Information

Background Information:

Xanthine oxidase (XOD) catalyzes the hydroxylation of many purine substrates and, in mammals, converts hypoxanthine to xanthine and then uric acid and O2-. The enzyme is widely distributed in mammals, and the abnormal product uric acid, which often causes gout, remains an issue of concern. In addition, the superoxide anion radical (O2-), generated upon the formation of uric acid, has been found to cause tissue damage. Inhibitors of this enzyme result in increased excretion of these purines and should be useful in reducing hyperurecemia which generally leads to gout.

Family:

Oxidoreductase

Sub Family:

Oxidase

Protein Name:

Xanthine Oxidase

Uniprot Number:

P47989

Gene Name:

XDH

Gene ID:

7498

Gene Aliases:

XOR|XO

Accession Number:

NM_173972

Organism:

Bovine

Construct Details:

Endogenous

Assay Information

Assay Type:

Biochemical

Assay Sub Type:

Enzymatic

Functional Mode:

Antagonist

Detection Method:

Spectrophotometry

Measured Response:

Absorbance

Testing Information

Procedure Summary:

Bovine milk xanthine oxidase is used. Test compound and/or vehicle is preincubated with 7 mU/ml enzyme‡ in phosphate buffer pH 7.5 for 15 minutes at 37⁰C. The reaction is initiated by addition of 0.165 mM xanthine for another 30 minute incubation period. Determination of the amount of uric acid formed is read spectrophotometrically. Compounds are screened at 10 μM.

Substrate:

165 µM Xanthine

Incubation:

30 min at 25°C

Control Inhibitor:

Allopurinol

Criteria For Significance:

≥ 50% of max stimulation or inhibition

Test Concentration / Dose:

Suggested concentration for initial testing is 10 uM

Test Sample Requirements:

Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).

Minimum Order Quantity:

2

Turnaround Time

Standard:

15 Business Days

Reference Compound Data

PubChem ID

2094

Name

Allopurinol

Measurement

2.4μM - IC50

Clinical Relevance

Therapeutic Area:

Metabolic Diseases

Additional Information

Brand:

LeadHunter

Testing Location:

Taiwan - Taipei

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