PTPRM Human Phosphatase Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
PTPRM (PTPRM, RPTPmu, RPTPU), a member of the protein tyrosine phosphatase (PTP) family, is thought to play an important role in cell-cell adhesion mediated processes. PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. The specific expression of PTPRM in arterial endothelial cells and cardiac myocytes suggests a possible role in the regulation of cardiovascular functions. PTPRM has been implicated in playing a tumour suppressor role in kidney, lung and breast cancers.
Family:
Phosphatase
Sub Family:
RTP
Class:
Protein Tyrosine
Protein Name:
PTPRM
Uniprot Number:
P28827
Gene Name:
PTPRM
Gene ID:
5797
Gene Aliases:
RPTPU|hR-PTPu
Accession Number:
NM_001105244
Organism:
Human
Construct Details:
Fragment (839-1100), Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human recombinant protein phosphatase RPTPm expressed in E. coli is used. Test compound and/or vehicle is preincubated with 62 ng/ml enzyme‡ for 15 minutes at 37°C in modified Tris-HCl buffer pH 8.5. The reaction is initiated by addition of 100 μM DiFMUP for 60 minutes. Determination of the amount of DiFMU formed is read spectrofluorimetrically at 358 nm/450 nm. Compounds are screened at 10 µM.
Substrate:
100 µM DiFMUP
Incubation:
60 min at 37°C
Control Inhibitor:
(NH₄)₆Mo₇O₂₄
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Adverse / Beneficial Action:
Inhibition of PTPRM may facilitate growth and metastasis of numerous cancers.
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
RELATED SOLUTIONS
Phosphatase Profiler LeadHunter Panel - TW
Item: PP260
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