PTPMEG2 Human Phosphatase Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
PTPN9 (protein tyrosine phosphatase, non-receptor type 9, MEG2) was originally cloned from human MEG-01 megakaryocyte and umbilical vein endothelial cell cDNA. The full-length enzyme has been reported to bind phospholipid with high affinity and that this binding is important for MEG2 activity on secretory vesicles. MEG2 is a polyphosphoinositide-activated tyrosine phosphatase that may be involved in signaling events regulating phagocytosis, essential antimicrobial function in the immune response, development of erythroid cells and insulin desensitization.
Family:
Phosphatase
Sub Family:
Non-Receptor
Class:
Protein Tyrosine
Protein Name:
PTPMEG2
Uniprot Number:
P43378
Gene Name:
PTPN9
Gene ID:
5780
Gene Aliases:
MEG2
Accession Number:
NM_002833
Organism:
Human
Construct Details:
Fragment (285-593), Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human recombinant protein phosphatase MEG2 expressed in E. coli is used. Test compound and/or vehicle is preincubated with 7.8 ng/ml enzyme‡ for 15 minutes at 37°C in modified Tris-HCl buffer pH 7.2. The reaction is initiated by addition of 100 μM DiFMUP for 60 minutes. Determination of the amount of DiFMU formed is read spectrofluorimetrically at 358 nm/450 nm. Compounds are screened at 10 µM.
Substrate:
100 µM DiFMUP
Incubation:
60 min at 37°C
Control Inhibitor:
(NH₄)₆Mo₇O₂₄
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Adverse / Beneficial Action:
Inhibition of MEG2 may facilitate microbial infections, reduce development of erythroid cells (especially those in polycythemia vera patients), and restore insulin sensitivity in type 2 diabetes.
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
RELATED SOLUTIONS
Phosphatase Profiler LeadHunter Panel - TW
Item: PP260
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