PTPMEG Human Phosphatase Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
PTPN4 (protein tyrosine phosphatase, non-receptor type 4, PTPMEG, MEG) is a non-transmembrane type protein tyrosine phosphatase that contains two src homology-2 (SH2) domains. The SH2 domains on PTP1C are believed to have dual roles: as facilitators promoting interactions between cytoplasmic signaling molecules and specific phosphotyrosyl residues on activated (i.e., autophosphorylated) growth factor receptors, and as self-regulators of phosphatase activity. PTPN4 is expressed predominantly in brain.
Family:
Phosphatase
Sub Family:
Non-Receptor
Class:
Protein Tyrosine
Protein Name:
PTPMEG
Uniprot Number:
P29074
Gene Name:
PTPN4
Gene ID:
5775
Gene Aliases:
PTPMEG
Accession Number:
NM_002830
Organism:
Human
Construct Details:
Fragment (637-926), Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human recombinant protein phosphatase PTPMEG expressed in E. coli is used. Test compound and/or vehicle is preincubated with 0.625 ng/ml enzyme‡ for 15 minutes at 37°C in modified Tris-HCl buffer pH 7.2. The reaction is initiated by addition of 1 μM DiFMUP for 60 minutes. Determination of the amount of DiFMU formed is read spectrofluorimetrically at 358 nm/450 nm. Compounds are screened at 10 µM.
Substrate:
1 µM DiFMUP
Incubation:
60 min at 37°C
Control Inhibitor:
(NH₄)₆Mo₇O₂₄
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Adverse / Beneficial Action:
Inhibition of PTPN4 may impair neuronal circuit formation, cell proliferation and adhesion.
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
RELATED SOLUTIONS
Phosphatase Profiler LeadHunter Panel - TW
Item: PP260
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