PTP-PEST Human Phosphatase Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
PTPN12 (Tyrosine-protein phosphatase non-receptor type 12, PTP-PESTPTPG1) is a ubiquitously expressed cytosolic protein tyrosine phosphatase. PTP-PEST contains the typical tyrosine phosphatase catalytic domain flanked by proline-rich regions that are shown to interact with several signaling proteins including Grb2, paxillin, p130CAS and other adhesion molecules. PTP-PEST is involved in negatively regulating specific aspects of T cell development.
Family:
Phosphatase
Sub Family:
Non-Receptor
Class:
Protein Tyrosine
Protein Name:
PTP-PEST
Uniprot Number:
Q05209
Gene Name:
PTPN12
Gene ID:
5782
Gene Aliases:
PTPG1|PTP-PEST
Accession Number:
NM_002835
Organism:
Human
Construct Details:
Fragment (2-300), Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human recombinant protein phosphatase PEST expressed in E. coli is used. Test compound and/or vehicle is preincubated with 2.8 ng/ml enzyme‡ for 15 minutes at 37°C in modified HEPES buffer pH 7.2. The reaction is initiated by addition of 100 μM DiFMUP for 60 minutes. Determination of the amount of DiFMU formed is read spectrofluorimetrically at 358 nm/450 nm. Compounds are screened at 10 µM.
Substrate:
100 µM DiFMUP
Incubation:
60 min at 37°C
Control Inhibitor:
(NH₄)₆Mo₇O₂₄
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Adverse / Beneficial Action:
Inhibition of PTP-PEST may reduce cell migration and spreading of fibroblasts resulting in impaired wound healing.
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
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