PP5 Human Phosphatase Enzymatical LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Protein phosphatase 5 (PP5) is a member of the PPP family of serine/threonine phosphatases, consists of a C-terminal catalytic domain and an N-terminal TPR (tetratricopeptide repeat) domain which is involved in protein-protein. PP5 has been implicated in wide ranging cellular processes including MAPK-mediated growth and differentiation, cell cycle arrest and DNA damage repair, regulation of ion channels, the cellular heat shock response and steroid receptor signaling.
Family:
Phosphatase
Sub Family:
Non-Receptor
Class:
Protein Ser/Thr
Protein Name:
PP5
Uniprot Number:
P53014
Protein Aliases:
protein phosphatase T
Gene Name:
PPP5C
Gene ID:
5536
Gene Aliases:
PP5
Accession Number:
NM_006247
Organism:
Human
Construct Details:
Full Length, Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human recombinant protein phosphatase PP5 expressed in E. coli cells is used. Test compound and/or vehicle is preincubated with 3.38 µg/ml enzymeǂ for 15 minutes at 37°C in modified HEPES buffer pH 7.2. The reaction is initiated by addition of 200 μM DiFMUP for 30 minutes. Determination of the amount of DiFMU formed is read spectrofluorimetrically at 358 nm/450 nm. Compounds are screened at 10 μM.
Substrate:
200 µM DiFMUP
Incubation:
30 min at 37°C
Control Inhibitor:
Nodularin
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
RELATED SOLUTIONS
Phosphatase Profiler LeadHunter Panel - TW
Item: PP260
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