PIK3R2 C-term Human Kinase SH2 Domain-Containing SH2scan KdELECT Binding LeadHunter Assay - US

Background Information:

Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (By similarity). (https://www.uniprot.org/uniprotkb/O00459/entry). SH2-domain binding assays were developed using Eurofins Discovery's proprietary KINOMEscan technology that utilizes DNA-tagged target proteins and a high sensitivity qPCR readout to enable highly sensitive (accurate ligand KDs measured down to low picomolar range) and quantitative competition binding assays.

Family:

Kinase

Sub Family:

PtdIns(4,5)P 3-Kinase

Class:

Lipid

Protein Name:

PIK3R2 (p85beta)

Uniprot Number:

O00459

Protein Aliases:

phosphoinositide-3-kinase regulatory subunit beta

Gene Name:

PIK3R2

Gene ID:

5296

Gene Aliases:

P85B|p85

Accession Number:

NP_005018.2

Organism:

Human

Construct Details:

Wild Type; Partial Length (Start/Stop:D610/R717); Mammalian Expression