Neutrophil Elastase Human Chymotrypsin Serine Peptidase Enzymatic LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Neurophil elastase 2 (PMN elastase, Leukocyte elastase, Bone marrow serine protease, Medullasin) is a serine protease that hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. Elastase 2 may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix.
Family:
Protease
Sub Family:
Chymotrypsin
Class:
Serine Peptidase
Protein Name:
Neutrophil Elastase
Uniprot Number:
P08246
Protein Aliases:
neutrophil elastase|leukocyte elastase|medullasin
Gene Name:
ELANE
Gene ID:
1991
Gene Aliases:
NE|HNE|HLE
Organism:
Human
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrophotometric
Testing Information
Substrate:
MeOSAAPV-pNa (0.4 mM)
Incubation:
30 min at 37°C
Control Inhibitor:
3',4'dichloroisocoumarin
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µl of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM final testing. 2) Dose Response: 90 µl of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
CNS/Addiction/Pain
Adverse / Beneficial Action:
Inhibition of elastase 2 may be useful in the treatment of degenerative and inflammatory diseases including emphysema, rheumatoid arthritis and psoriasis.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
Questions? Contact Us
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