OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Micronucleus formation is a hallmark of genotoxicity, and the micronucleus assay is an important component of genotoxicity screening. Micronuclei are chromatin-containing bodies that represent fragments or even whole chromosomes that were not incorporated into a daughter cell nucleus at mitosis. The purpose of the assay is to detect agents that induce chromosome damage leading to the induction of micronuclei in interphase cells. The in vitro micronucleus assay is conducted in CHO-K1 cells. The formation of the micronucleus is identified by image-base high content analysis from binucleated cells.
Organism:
Hamster
Cell Type:
CHO-K1
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Detection Method:
Imaging / HCA
Platform:
MD ImageXpress® Micro
Measured Response:
Fluorescence
Testing Information
Incubation:
24 hr at 37C
Criteria For Significance:
p < 0.05
Test Concentration / Dose:
Assay is performed with 8 compound concentration(s) and each concentration tested using 2 replicate
Test Sample Requirements:
Minimum for 1) Screen: 60 μl of 100 mM stock -OR- 3 mg (pre-weighed).
Turnaround Time
Standard:
10 Business Days
Additional Information
Brand:
Cerep
Testing Location:
USA - St Charles, MO
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