Lambda PP Phage Phosphatase Enzymatical LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Lambda protein phosphatase is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues. The bacteriophage lambda enzyme exhibited a high degree of similarity to the catalytic subunits of a variety of protein serine/threonine phosphatases, many of which have recently been shown to play critical roles in diverse cellular processes such as growth and differentiation, oncogenesis, DNA replication, transcription, and protein synthesis.
Family:
Phosphatase
Sub Family:
Non-Receptor
Class:
Protein Ser/Thr
Protein Name:
Lambda PP
Uniprot Number:
P03772
Gene Name:
N/A
Gene ID:
N/A
Gene Aliases:
N/A
Accession Number:
NC_001416
Organism:
Phage
Construct Details:
Fragment (2-end), Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Recombinant protein phosphatase lPP expressed in E. coli cells is used. Test compound and/or vehicle is preincubated with 0.002 μg/ml enzymeǂ for 15 minutes at 37°C in modified HEPES buffer pH 7.2. The reaction is initiated by addition of 10 μM DiFMUP for 30 minutes. Determination of the amount of DiFMU formed is read spectrofluorimetrically at 358 nm/450 nm. Compounds are screened at 10 μM.
Substrate:
10 µM DiFMUP
Incubation:
30 min at 37°C
Control Inhibitor:
mpV
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Autoimmune
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
RELATED SOLUTIONS
Phosphatase Profiler LeadHunter Panel - TW
Item: PP260
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