ITK Human Kinase SH2 Domain-Containing SH2scan ELECT Binding LeadHunter Assay - US

Background Information:

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. (https://www.uniprot.org/uniprotkb/Q08881/entry). SH2-domain binding assays were developed using Eurofins Discovery's proprietary KINOMEscan technology that utilizes DNA-tagged target proteins and a high sensitivity qPCR readout to enable highly sensitive (accurate ligand KDs measured down to low picomolar range) and quantitative competition binding assays.

Family:

Kinase

Sub Family:

TK

Class:

Protein Tyrosine

Protein Name:

ITK

Uniprot Number:

Q08881

Protein Aliases:

Interleukin-2-inducible T-cell kinase|IL-2-inducible T-cell kinase|Kinase EMT|T-cell-specific kinase|Tyrosine-protein kinase Lyk

Gene Name:

ITK

Gene ID:

3702

Gene Aliases:

EMT|PSCTK2|LYK

Accession Number:

NP_005537.3

Organism:

Human

Construct Details:

Wild Type; Partial Length (Start/Stop:N232/C339); Mammalian Expression