HePTP Human Phosphatase Enzymatical LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Hematopoietic protein tyrosine phosphatase (HeTP) protein, also called protein tyrosine phosphatase non-receptor type 7, PTPN7, LC-PTP, LPTP, is a cytosolic enzyme expressed strongly in T cells. HePTP plays a negative role signaling by down-regulating MAPK activity and responsible for many different types of signaling including reaction to stress and inflammation and cell migration. Amplification and overexpression of HePTP may be an important cofactor contributing to abnormal myeloid cell growth.
Family:
Phosphatase
Sub Family:
Non-Receptor
Class:
Protein Tyrosine
Protein Name:
HePTP
Uniprot Number:
P35236
Gene Name:
PTPN7
Gene ID:
5778
Gene Aliases:
HEPTP|LC-PTP
Accession Number:
NM_002832
Organism:
Human
Construct Details:
Fragment (22-end), Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human recombinant protein phosphatase HePTP expressed in E. coli cells is used. Test compound and/or vehicle is preincubated with 0.016 μg/ml enzymeõ for 15 minutes at 37°C in modified HEPES buffer pH 7.2. The reaction is initiated by addition of 10 μM DiFMUP for 30 minutes. Determination of the amount of DiFMU formed is read spectrofluorimetrically at 358 nm/450 nm. Compounds are screened at 10 μM.
Substrate:
10 µM DiFMUP
Incubation:
30 min at 37°C
Control Inhibitor:
(NH₄)₆Mo₇O₂₄
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Autoimmune
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
RELATED SOLUTIONS
Phosphatase Profiler LeadHunter Panel - TW
Item: PP260
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