Glucuronide conjugate detection (liver microsomes, rat, Sprague-Dawley) - US
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
This protocol is designed to detect qualitatively if the test compound and/or its hydroxylated metabolite(s) forms a conjugate with glucuronic acid in liver microsomes from human or animal species.
Organism:
Rat
Construct Details:
Microsomes
Tissue:
Liver
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Detection Method:
HPLC-MS
Measured Response:
Peak Area Response
Testing Information
Substrate:
Test compound
Test Concentration / Dose:
Assay is performed with each concentration tested using 2 replicate(s)
Test Sample Requirements:
Minimum for 1) Screen: 100 μl of 10 mM stock -OR- 1 to 2 mg (pre-weighed). 2) Dose Response: 250 μl of 10 mM stock -OR- 1.5 to 2 mg (pre-weighed).
Turnaround Time
Standard:
15 Business Days
Additional Information
Brand:
Cerep
Testing Location:
USA - St Charles, MO
Other Information:
Client to provide compound chemical structure to ClientServicesStCharles@discovery.eurofinsus.com prior to study initiation of this assay.
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