Glucokinase (GCK) Human Hexokinase Enzymatic Activation LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Glucokinase catalyzes the formation of glucose-6-phosphate, expressed in the pancreatic beta cell and liver involved in sensing systemic glucose and regulating glucose homeostasis.
Family:
Kinase
Protein Name:
GCK
Uniprot Number:
P35557
Protein Aliases:
Glucokinase
Accession Number:
NM_000162
Organism:
Human
Construct Details:
Fragment (16-465), Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Agonist
Detection Method:
Spectrophotometry
Platform:
Spectrofluorimetry
Testing Information
Procedure Summary:
Human recombinant Glucokinase (GCK) expressed in E. coli cells is used. Test compound or 100 µM PF 04991532 or vehicle is preincubated with 4 µg/ml enzyme in modified HEPES buffer pH 7.0 for 15 minutes at 25C. The reaction is initiated by addition of 20 mM Glucose, 200 µM ATP and 2 µg/ml Coupling Phosphatase 4 for another 20 minute incubation period. The amount of inorganic phosphate are determined spectrophotometrically at 620 nm by malachite green reagent. Compounds are screened at 10 µM.
Substrate:
20 µM Glucose
Incubation:
20 min at 25°C
Control Inhibitor:
PF 04991532
Criteria For Significance:
≥ 50% of max stimulation
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
10
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
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