Free Radical Scavenger Synthetic DPPH Radical Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
A free radical is an atom or molecule that has one or more unpaired electrons. The evidence is that reactive species of oxygen (O2-, H2O2, OH- and NO2+) play an important role in pulmonary oxygen toxicity, inflammation, neurodegenerative disorders, ischemia/reperfusion injury and cancer, etc. Antioxidants can react with the nitrogen centered radical DPPH (1,1-diphenyl-2-picryl-hydrazyl) and convert it to the non-radical 1,1-diphenyl-2-picrylhydrazine.
Family:
Free Radical Scavenger
Organism:
Synthetic
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrophotometry
Measured Response:
Absorbance
Testing Information
Procedure Summary:
Test compound and/or vehicle is incubated with DPPH solution for 5 minutes at 25⁰C. Determination of scavenger activity is determined spectrophotometrically by quenching the DPPH radical. Compounds are screened at 10 μM.
Substrate:
250 μM DPPH+
Incubation:
5 min at 25°C
Control Inhibitor:
TroloxTM C
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
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