Free Radical Scavenger Bovine SOD Mimetic Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
The evidence that reactive species of oxygen (O2-, H2O2 and OH-) play an important role in pulmonary oxygen toxicity, inflammation, ischemia/reperfusion injury, etc., and that superoxide dismutase (SOD, EC.1.15.1.1) provides a defense against superoxide radicals, increases continuously. This enzyme catalyzes the dismutation of two superoxide radicals (O2-) into O2 and H2O2. Many methods have been devised to measure superoxide dismutase activity; the method described below is based on the inhibition of nitroblue tetrazolium reduction with xanthine-xanthine oxidase used as a superoxide generator. Agents that decrease superoxide O2- formation may have therapeutic potential for treatment of the above noted disease states.
Family:
Free Radical Scavenger
Accession Number:
NM_173972
Organism:
Bovine
Construct Details:
Endogenous
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrophotometry
Measured Response:
Absorbance
Testing Information
Procedure Summary:
Test compound and/or vehicle is incubated with 0.12 mM xanthine, 3 mU/ml‡ xanthine oxidase and 27 μM nitroblue tetrazolium in Na2CO3 solution at pH 10.5 for 20 minutes at 25⁰C. Determination of formazan formed is read spectrophotometrically at 595 nm. Compounds are screened at 10 μM.
Substrate:
120 µM Xanthine
Incubation:
20 min at 25°C
Control Inhibitor:
Superoxide dismutase (SOD)
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
CNS/Addiction/Pain
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
RELATED SOLUTIONS
Metabolic Dysfunction-Associated Steatohepatitis (MASH) LeadHunter Panel - TW
Item: PP318
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