Factor VIIa Human Chymotrypsin Serine Peptidase Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Human factor VIIa is a vitamin K-dependent plasma serine protease generated by proteolysis of zymogen FVII. FVIIa binds with high affinity to tissue factor forming the tissue factor/Factor VIIa complex. This complex with factor VIIa as the catalytic subunit initiates the extrinsic coagulation pathway by activation of factor X, factor IX and additional factor VII.
Family:
Protease
Sub Family:
Chymotrypsin
Class:
Serine Peptidase
Protein Name:
Factor VIIa
Uniprot Number:
P08709
Protein Aliases:
Proconvertin; Serum prothrombin conversion accelerator (SPCA)
Gene Name:
F7
Gene ID:
2155
Gene Aliases:
eptacog alfa; FVII coagulation protein; factor VII
Accession Number:
NM_000131
Organism:
Human
Construct Details:
Endogenous
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human plasma coagulation factor VIIa is used. Test compound and/or vehicle is preincubated with 3 μg/mL enzyme in modified 50 mM Tris-HCl buffer pH 7.6 for 15 minutes at 25˚C. The reaction is initiated by addition of 100 μM Boc-LTR-AMC for another 60-minute incubation period. Determination of the amount of AMC formed is read spectrofluorometrically at excitation 360 nm and emission 465 nm. Compounds are screened at 10 μM.
Substrate:
100 μM Boc-LTR-AMC
Incubation:
60 min at 25°C
Control Inhibitor:
PCI-27483
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 μM
Test Sample Requirements:
Minimum for 1) Screen: 25 μL of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μL of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
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