EP4 Human Prostanoid GPCR Cell Based Agonist Arrestin LeadHunter Assay - US

Background Information:

Prostanoid receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Prostanoid EP4 receptors are present in brain and many peripheral tissues (e.g., ileum>thymus>spleen>lung>heart>stomach).

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Family:

GPCR

Sub Family:

Prostanoid

Class:

Class A

Coupling:

Gs

Protein Name:

EP4

Uniprot Number:

P35408

Protein Aliases:

PGE2 receptor EP4 subtype|Prostanoid EP4 receptor

Gene Name:

PTGER4

Gene ID:

5734

Gene Aliases:

EP4

Accession Number:

AY429109

Organism:

Human

Cell Type:

HEK293