CDC25B Human Phosphatase Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Cell Division Cycle 25B (CDC25B) is a member of the CDC25 family of dual specificity protein tyrosine phosphatases that dephosphorylate and activate cyclin-dependent kinases (CDKs). Mammalian cells express at least three dual phosphatase Cdc25 homologues, named A, B, and C. CDC25B has been proposed to activate the CDK1/cyclin B1 complex to advance the cell cycle from G2 to M.
Family:
Phosphatase
Sub Family:
Dual Specificity
Class:
Protein Tyrosine
Protein Name:
CDC25B
Uniprot Number:
P30305
Gene Name:
CDC25B
Gene ID:
994
Accession Number:
NM_021873
Organism:
Human
Construct Details:
Full Length, Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human recombinant protein phosphatase CDC25B expressed in E. coli is used. Test compound and/or vehicle is preincubated with 0.61 μg/ml enzyme‡ for 15 minutes at 37°C in modified Tris-HCl buffer pH 8.5. The reaction is initiated by addition of 15 μM 3-O-methylfluorescein phosphate for 60 minutes. Determination of the amount of 3-O-methylfluorescein formed is read spectrofluorimetrically at 475 nm/530 nm. Compounds are screened at 10 µM.
Substrate:
15 µM 3-O-methylfluorescein phosphate
Incubation:
60 min at 37°C
Control Inhibitor:
NSC 663284
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Adverse / Beneficial Action:
CDC25 is an oncogene and its inhibition may be useful in reducing tumor cell proliferation.
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
Questions? Contact Us
You can easily get in touch with a Eurofins Discovery Expert or get help from our Customer Service team. Reach out with questions, or to discuss your specific discovery challenges.
