BB2 Human Bombesin GPCR Cell Based PAM Arrestin LeadHunter Assay - US

Background Information:

Bombesin receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Bombesin receptors are widely distributed in the central nervous system and peripheral tissues. Bombesin BB2 (gastrin-releasing peptide, GRP) receptor agonism may promote gastric emptying, gastrointestinal transit, insulin secretion, inflammation, cell proliferation and tumors. Receptor antagonism may lead to abnormal social behavior.

PathHunter β-Arrestin GPCR cell lines are engineered to co-express the ProLink™ (PK) tagged GPCR and the Enzyme Acceptor (EA) tagged β-Arrestin. Activation of the GPCR-PK induces β-Arrestin-EA recruitment, forcing complementation of the two enzyme fragments. The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. PathHunter cell lines expressing Gq-coupled receptors can also be used to detect calcium mobilization.

Family:

GPCR

Sub Family:

Bombesin

Class:

Class A

Protein Name:

BB2

Uniprot Number:

P30550

Protein Aliases:

bombesin receptor 2

Gene Name:

GRPR

Gene ID:

2925

Gene Aliases:

BB2|BB2R

Accession Number:

NM_005314

Organism:

Human

Cell Type:

U2OS