ACE2 Human Angiotensin-Converting Metallo Peptidase Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Angiotensin-converting enzyme 2 (ACE2), identified human homolog of ACE, is a novel metalloprotease with specificity, tissue distribution (heart, lungs and kidneys), and function distinct from those of ACE. ACE2 may play a unique role in the renin-angiotensin system and mediate cardiovascular and renal function. Inhibitors of ACE2-specific peptide may be useful in the treatment of hypertension and other cardiovascular diseases.
Family:
Protease
Sub Family:
Angiotensin-Converting
Class:
Metallo Peptidase
Protein Name:
ACE2
Uniprot Number:
Q9BYF1
Protein Aliases:
peptidyl-dipeptidase A
Gene Name:
ACE2
Gene ID:
59272
Accession Number:
NM_001371415
Organism:
Human
Construct Details:
Fragment (18-740), Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human recombinant angiotensin converting enzyme-2 expressed in mouse myeloma cell line is used. Test compound and/or vehicle are preincubated with 45 ng/ml‡ of enzyme for 15 minutes at 25⁰C in Tris buffer pH 7.4. The reaction is initiated by addition of 10 µM of Mca-YVADAPK(Dnp)-OH for another 20 minute incubation period. Read plate on a spectrophotometer (Excitation: 340 nm, Emission: 430 nm). Compounds are screened at 10 μM.
Substrate:
10 μM Mca-Tyr-Val-Ala-Asp-Pro-Ala-Lys-(DNP)-OH
Incubation:
20 min at 25°C
Control Inhibitor:
Ac-GG-26-NH2
Criteria For Significance:
≥ 50 of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Cardiovascular
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
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