Time-dependent CYP2C9 Inhibition (HLM; diclofenac substrate) - US
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Cytochrome P450 (CYP) inhibition is one of the most common types of drug-drug interactions (DDI) and can be exhibited in either a reversible or time-dependent manner. This assay is designed to determine if a test compound is an irreversible, time-dependent inhibitor of CYP2C9 in human liver microsomes
No. of Assays on Panel:
2
Assays on Panel:
Family
CYP450
Name
Time-dependent CYP2C9 inhibition (HLM, diclofenac substrate + NADPH) - US
Item
2544
Protein
CYP2C9
Organism
Human
Assay Sub Type
Enzymatic
Family
CYP450
Name
Time-dependent CYP2C9 inhibition (HLM, diclofenac substrate - NADPH) - US
Item
2545
Protein
CYP2C9
Organism
Human
Assay Sub Type
Enzymatic
Family:
CYP450
Sub Family:
CYP2
Protein Name:
CYP2C9
Uniprot Number:
P11712
Gene Name:
CYP2C9
Gene ID:
1559
Gene Aliases:
P450IIC9
Organism:
Human
Construct Details:
Microsomes
Tissue:
Liver
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
HPLC-MS/MS
Measured Response:
Peak Area Response
Testing Information
Substrate:
diclofenac
Test Concentration / Dose:
Assay is performed with each concentration tested using 2 replicate(s)
Test Sample Requirements:
Minimum for 1) Screen: 100 μl of 10 mM stock -OR- 1 to 2 mg (pre-weighed). 2) Dose Response: 250 μl of 10 mM stock -OR- 1.5 to 2 mg (pre-weighed).
Turnaround Time
Standard:
10 Business Days
Additional Information
Brand:
Cerep
Testing Location:
USA - St Charles, MO
RELATED SOLUTIONS
CYP2C9 inhibition (recombinant, MFC substrate) - US
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Intrinsic clearance (CYP2C9) - US
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CYP2C9 inhibition (HLM, diclofenac substrate) - US
Item: 2066
CYP2C9 inhibition (recombinant, diclofenac substrate) - US
Item: 2067
Time-dependent CYP2C9 inhibition (HLM, diclofenac substrate + NADPH) - US
Item: 2544
Time-dependent CYP2C9 inhibition (HLM, diclofenac substrate - NADPH) - US
Item: 2545
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