TRH Human Thyrotropin-RH GPCR Cell Based Agonist & Antagonist Calcium Flux LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Thyrotropin releasing hormone (TRH) receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. TRH receptors are widely and regionally distributed throughout the neuraxis.
No. of Assays on Panel:
2
Assays on Panel:
Family
GPCR
Name
TRH Human Thyrotropin-RH GPCR Cell Based Agonist Calcium Flux LeadHunter Assay - FR
Item
1617
Protein
TRH
Organism
Human
Assay Sub Type
Cell Based
Family
GPCR
Name
TRH Human Thyrotropin-RH GPCR Cell Based Antagonist Calcium Flux LeadHunter Assay - FR
Item
2057
Protein
TRH
Organism
Human
Assay Sub Type
Cell Based
Family:
GPCR
Sub Family:
Thyrotropin-Releasing Hormone
Class:
Class A
Protein Name:
TRH
Uniprot Number:
P34981
Protein Aliases:
Thyroliberin receptor
Gene Name:
TRHR
Gene ID:
7201
Organism:
Human
Cell Type:
CHO-K1
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Agonist & Antagonist
Detection Method:
Fluorimetry
Measured Response:
Fluorescence
Testing Information
Incubation:
RT
Control Activator:
TRH (1 nM)
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 70 µL of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM finµL testing. 2) Dose Response: 90 µL of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
16
Turnaround Time
Standard:
15 Business Days
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Adverse / Beneficial Action:
TRH receptor agonism may stimulate synthesis and release of thyroid stimulating hormone (TSH), augment autonomic tone, cause hyperprolactinenia and galactorrhea; restoration of conciousness and mobility in anesthetized animals is well documented.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
TRH Human Thyrotropin-RH GPCR Cell Based Agonist Calcium Flux LeadHunter Assay - FR
Item: 1617
TRH Human Thyrotropin-RH GPCR Cell Based Antagonist Calcium Flux LeadHunter Assay - FR
Item: 2057
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