A2B Human Adenosine GPCR Cell Based Agonist & Antagonist cAMP LeadHunter Assay - FR
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Adenosine receptors belong to the superfamily of G protein-coupled seven transmembrane proteins. G-protein-coupled receptors constitute one of the major signal transduction systems in eukaryotic cells. Coding sequences for these receptors, in those regions believed to contribute to the agonist-antagonist binding site, are strongly conserved across mammalian species. Adenosine receptors may also be called P1 receptors, which are distinguished from transmitter-gated ion channels (P2X receptors) and G protein-coupled receptors (P2Y). Adenosine A2B receptor agonism may cause vascular and intestinal smooth muscle relaxation, antagonism may be useful in the treatment of pulmonary inflammation associated with asthma.
No. of Assays on Panel:
2
Assays on Panel:
Family
GPCR
Name
A2B Human Adenosine GPCR Cell Based Agonist cAMP LeadHunter Assay - FR
Item
452
Protein
A2B
Organism
Human
Assay Sub Type
Cell Based
Family
GPCR
Name
A2B Human Adenosine GPCR Cell Based Antagonist cAMP LeadHunter Assay - FR
Item
455
Protein
A2B
Organism
Human
Assay Sub Type
Cell Based
Family:
GPCR
Sub Family:
Adenosine
Class:
Class A
Protein Name:
A2B
Uniprot Number:
P29275
Gene Name:
ADORA2B
Gene ID:
136
Organism:
Human
Cell Type:
HEK293
Assay Information
Assay Type:
Functional
Assay Sub Type:
Cell Based
Functional Mode:
Agonist & Antagonist
Detection Method:
HTRF
Testing Information
Incubation:
10 min at 37°C
Control Activator:
NECA (1000 nM)
Test Concentration / Dose:
Eurofins will provide IC/EC50 calculation if 5 or more concentrations are selected.
Test Sample Requirements:
Minimum for 1) Screen: 60 µL of 10 mM stock -OR- 1 mg (pre-weighed) for 10µM finµL testing. 2) Dose Response: 90 µL of 10 mM stock -OR- 1 mg (pre-weighed) for a top concentration at 10 µM.
Minimum Order Quantity:
16
Turnaround Time
Standard:
15 Business Days
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Adverse / Beneficial Action:
Adenosine A2B receptor agonism may cause vascular and intestinal smooth muscle relaxation, antagonism may be useful in the treatment of pulmonary inflammation associated with asthma.
Additional Information
Brand:
LeadHunter
Testing Location:
France - Celle L'Evescault
RELATED SOLUTIONS
A2B Human Adenosine GPCR Binding Antagonist Radioligand LeadHunter Assay - FR
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A2B Human Adenosine GPCR Cell Based Agonist cAMP LeadHunter Assay - FR
Item: 452
A2B Human Adenosine GPCR Cell Based Antagonist cAMP LeadHunter Assay - FR
Item: 455
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