CYP2B6 induction (human hepatocytes, enzyme activity, 1 donor) - US

Item: 2383

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OVERVIEW

DOCUMENTS/REFERENCES

Biological Information

Background Information:

Some chemicals can induce the activity of CYP enzymes. These inducers will increase the metabolism of the co-administered drugs that are substrates of the induced CYP enzymes, resulting in these drugs losing efficacy. Eurofins in vitro CYP induction assay allow identification of compounds that may induce CYP activities, causing potential drug-drug interactions. The assays are performed using plateable cryopreserved human primary hepatocytes. The CYP activity is determined using the respective substrate of each CYP isozyme. The corresponding metabolite is detected by HPLC-MS/MS methods. The negative control and positive controls are included in each assay.

Family:

CYP450

Sub Family:

CYP2

Protein Name:

CYP2B6

Uniprot Number:

P20813

Gene Name:

CYP2B6

Gene ID:

1555

Gene Aliases:

CPB6|CYPIIB6

Organism:

Human

Construct Details:

Endogenous

Tissue:

Liver

Cell Type:

Hepatocytes

Assay Information

Assay Type:

Functional

Assay Sub Type:

Cell Based

Functional Mode:

Agonist

Detection Method:

HPLC-MS/MS

Measured Response:

Peak Area Response

Testing Information

Substrate:

200 µM Bupropion

Incubation:

72 hr at

Test Concentration / Dose:

Assay is performed with 1 compound concentration(s) and each concentration tested using 2 replicate

Test Sample Requirements:

Minimum for 1) Screen: 100 µl of 10 mM stock -OR- 1 to 2 mg (pre-weighed). 2) Dose Response: 250 µl of 10 mM stock -OR- 1.5 to 2 mg (pre-weighed).

Turnaround Time

Standard:

20 Business Days

Clinical Relevance

Adverse / Beneficial Action:

Inhibition may result in altered drug metabolism.

Additional Information

Brand:

Cerep

Testing Location:

USA - St Charles, MO