JAK3 Human TK Kinase Enzymatic Radiometric LeadHunter Assay [10 μM ATP] - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
Janus kinase 3 (JAK3) is a nonreceptor tyrosine kinase belonging to the Janus family. It is activated by a variety of cytokine and growth factor receptors, resulting in signaling cascades that facilitate the activation of various downstream target genes. JAK3 mediates gene transcription through its well-characterized downstream signaling molecules, the signal transducers and activators of transcription proteins (STAT). Inhibition of JAK3 may impair erythropoietin (EPO)-induced erythropoeiesis and protection from bain damage following hypoxia. Inhibition may also adversely effect diabetes.
Family:
Kinase
Sub Family:
TK
Class:
Protein Tyrosine
Protein Name:
JAK3
Uniprot Number:
P52333
Protein Aliases:
tyrosine-protein kinase JAK3|leukocyte Janus kinase
Gene Name:
JAK3
Gene ID:
3718
Gene Aliases:
L-JAK|JAKL|LJAK|JAK3_HUMAN|JAK-3
Accession Number:
NM_000215
Organism:
Human
Construct Details:
Fragment (781-1124), Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Radiometry
Measured Response:
Scintillation
Testing Information
Procedure Summary:
Human recombinant protein kinase JAK3 expressed in insect cells is used. Test compound and/or vehicle is preincubated with 0.6 µg/ml enzyme‡ for 15 minutes at 37°C in modified HEPES buffer pH 7.4. The reaction is initiated by addition of 0.2 mg/ml poly(Glu:Tyr), 10 µM ATP containing 0.25 µCi [γ32P]ATP for 30 minutes and terminated by further addition of 3% H3PO4. An aliquot is counted to determine the radioactivity of [32P]Poly(Glu:Tyr). Compounds are screened at 10 µM.
Substrate:
200 µg/ml Poly(Glu:Tyr)
ATP Concentration:
10 uM
Incubation:
30 min at 37°C
Control Inhibitor:
Staurosporine
Criteria For Significance:
≥ 50% of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Test Sample Requirements:
Minimum for 1) Screen: 25 μl of 10 mM stock -OR- 1 mg (pre-weighed). 2) Dose Response: 60 μl of 10 mM stock -OR- 1 mg (pre-weighed).
Minimum Order Quantity:
10
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
RELATED SOLUTIONS
JAK2 Human TK Kinase Enzymatic Radiometric LeadHunter Assay [10 μM ATP] - TW
Item: 174070
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