15-LOX-2 Human Lipoxygenase Enzymatic LeadHunter Assay - TW
OVERVIEW
DOCUMENTS/REFERENCES
Biological Information
Background Information:
15-Lipoxygenase initiates the metabolic pathway leading to the formation of lipoxins by converting arachidonic acid to 15-hydroxyperoxy-eicosatetraenoic acid (15-HPETE). Lipoxins may share some of the bioactivity associated with leukotrienes.
Family:
Lipid Metabolism
Sub Family:
Eicosanoid Turnover
Class:
Lipoxygenase
Protein Name:
15-LOX-2
Uniprot Number:
O15296
Protein Aliases:
arachidonate 15-lipoxygenase B, 15-LOX-B
Gene Name:
ALOX15B
Gene ID:
247
Gene Aliases:
15-LOX-2
Accession Number:
NM_001039130
Organism:
Human
Construct Details:
Full Length, Recombinant
Assay Information
Assay Type:
Biochemical
Assay Sub Type:
Enzymatic
Functional Mode:
Antagonist
Detection Method:
Spectrofluorimetry
Measured Response:
Fluorescence
Testing Information
Procedure Summary:
Human recombinant 15-LO expressed in E. coli cells is used. Test compound or vehicle are combined with 16 µg/mlǂ of enzyme for 15 minutes at 37°C in Tris buffer pH 7.4. The reaction is initiated by addition of 10 µM arachidonyl amide and dye DHR123 for 30 minutes at 37°C. Read plate on a spectrophotometer (Excitation: 485 nm, Emission: 535 nm). Compounds are screened at 10 µM.
Substrate:
10 µM AMC Arachidonoyl Amide
Incubation:
30 min at 37°C
Control Inhibitor:
2-TEDC
Criteria For Significance:
≥ 50 of max stimulation or inhibition
Test Concentration / Dose:
Suggested concentration for initial testing is 10 uM
Minimum Order Quantity:
2
Turnaround Time
Standard:
15 Business Days
Reference Compound Data
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Additional Information
Brand:
LeadHunter
Testing Location:
Taiwan - Taipei
Questions? Contact Us
You can easily get in touch with a Eurofins Discovery Expert or get help from our Customer Service team. Reach out with questions, or to discuss your specific discovery challenges.
