Calcium Flux Assay

HTS182C - ChemiSCREEN™ Human Recombinant PK2 Prokineticin Receptor Calcium-Optimized Stable Cell Line

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HTS182C
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    Prokineticins, also known as endocrine gland vascular endothelial growth factors (EG-VEGF), are two ~10 kD secreted proteins originally described to mediate angiogenesis and gastrointestinal smooth muscle contraction (Li et al., 2001; LeCouter et al., 2003). Subsequently, prokineticins have been found to mediate central nervous system functions including circadian rhythms and olfactory bulb development (Cheng et al., 2002; Ng et al., 2005). Two Gq-coupled receptors, PK1 and PK2 (also known as GPR73a and GPR73b), mediate cellular responses to prokineticins (Lin et al., 2002). The phenotype of the PK2-null mouse indicates that PK2 promotes development of the olfactory bulb and several male and female reproductive organs (Matsumoto et al., 2006). The cloned human PK2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PK2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PK2 and its ligands.

    Additional Resource :  HTS182C060315 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 mL per vial.
    Storage: On receipt of material store at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human PK2 cDNA (Accession Number: NM_144773- see CODING SEQUENCE below) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Cheng MY et al. (2002) Prokineticin 2 transmits the behavioural circadian rhythm of the suprachiasmatic nucleus. Nature 417: 405-10.
    Reference 2: 2. LeCouter J et al. (2003) Endocrine gland-derived VEGF and the emerging hypothesis of organ-specific regulation of angiogenesis. Nat. Med. 8: 913-7.
    Reference 3: 3. Li M et al. (2001) Identification of two prokineticin cDNAs: recombinant proteins potently contract gastrointestinal smooth muscle. Mol. Pharmacol. 59: 692-8.
    Reference 4: 4. Lin DC et al. (2002) Identification and molecular characterization of two closely related G protein-coupled receptors activated by prokineticins/endocrine gland vascular endothelial growth factor. J. Biol. Chem. 277: 19276-80.
    Reference 5: 5. Matsumoto S et al. (2006) Abnormal development of the olfactory bulb and reproductive tissues in mice lacking prokineticin receptor PKR2. Proc. Natl. Acad. Sci. USA 103: 4140-4145.
    Reference 6: 6. Ng KL et al. (2005) Dependence of olfactory bulb neurogenesis on prokineticin 2 signaling. Science 308: 1923-7.
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