Calcium Flux Assay

HTS160C - ChemiScreen™ Human Recombinant BB3 Bombesin Receptor Calcium-Optimized Stable Cell Line

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    Bombesin, a bioactive peptide first identified in amphibian skin, is related to two mammalian peptides, gastrin-releasing peptide (GRP) and neuromedin B. A family of 3 GPCRs, including GRP-R (BB1), NMB-R (BB2) and BRS-3 (BB3), mediate the biological effects of the peptides (Ohki-Hamazaki et al., 2005). BB3 differs from the others by its low affinity for bombesin. Although an endogenous ligand for BB3 has yet to be identified, a synthetic nonselective bombesin-like peptide [D-Phe6, β-Ala11,Phe13,Nle14]-bombesin-(6-14)-amide activates BB3 with high potency. BB3-null mice develop mild obesity that can be alleviated by treatment with an antiobesity compound, the serotonin and noradrenaline reuptake inhibitor Sibutramine (Matsumoto and Iijima, 2003). The cloned human BB3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant BB3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at BB3.

    Additional Resource :  HTS160C050415 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 mL per vial.
    Storage: Vials are to be stored in liquid N2. Media Component at 4ºC (-20ºC for prolonged storage).
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human BB3 cDNA (Accession Number: NM_001727- see CODING SEQUENCE below) and promiscuous G protein expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Matsumoto K and Iijima H (2003) Sibutramine sensitivity assay revealed a unique phenotype of bombesin BB3 receptor-deficient mice. Eur. J. Pharmacol. 473: 41-46.
    Reference 2: 2. Ohki-Hamazaki H et al. (2005) Development and function of bombesin-like peptides and their receptors. Int. J. Dev. Biol. 49: 293-300.