Calcium Flux Assay

HTS139C - ChemiSCREEN™ Human Recombinant SST5 Somatostatin Receptor Calcium-Optimized Stable Cell Line

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    Somatostatin (sst) is a multifunctional peptide with two biologically active forms, sst-14 and sst-28, which are synthesized in neurons throughout the brain as well as in peripheral tissues such as the pancreas and the gut (Gillies, 1997). SST exerts a diverse array of effects that include inhibition of endocrine secretion, modulation of neurotransmission, and regulation of cell proliferation by stimulating a family of five G-protein-coupled receptors. Somatostatin receptor sst5 is an inhibitory G protein-coupled receptor that exerts a strong cytostatic effect on various cell types. In mice, SST5 mediates somatostatin inhibition of pancreatic insulin secretion and contributes to the regulation of glucose homeostasis and insulin sensitivity (Strowski et al., 2003). In addition, deficiency of SST5 leads to subtype-selective sexually dimorphic changes in the expression of both brain and pancreatic somatostatins (Ramirez et al., 2004). The cloned human sst5-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant SST5 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between SST5 and its ligands.

    Additional Resource :  HTS139C060515 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 mL per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human SST5 cDNA (Accession Number: NM_001053.1- see CODING SEQUENCE below) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Gillies G (1997) Somatostatin: the neuroendocrine story. Trends Pharmacol. Sci. 18: 87-95.
    Reference 2: 2. Strowski, MZ, Kohler, M., Chen, HY et al. (2003). Somatostatin receptor subtype 5 regulates insulin secretion and glucose homeostasis. Mol. Endocrinol. 17: 93–106.
    Reference 3: 3. Ramirez, JL., Grant, M., Norman, M. et al. (2004) Deficiency of somatostatin (SST) receptor type 5 (SSTR5) is associated with sexually dimorphic changes in the expression of SST and SST receptors in brain and pancreas. Mol. Cell. Endocrinol. 221: