Calcium Flux Assay

HTS126C - ChemiScreen™ Human Recombinant TRH Thyrotropin-Releasing Hormone Receptor Calcium-Optimized Stable Cell Line

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    Thyrotropin-releasing hormone (TRH) is a tripeptide (L-pyroglutamyl-L-histidinyl-L-proline amide) that is released from the hypothalamus to stimulate release of thyroid-stimulating hormone from the thyrotropes of the anterior pituitary. In addition, TRH is synthesized in other regions of the CNS, where it appears to function as a neuromodulator to influence arousal, cognition, motor activity, depression, and food and water intake. TRH functions in the autonomic nervous system to modulate gastrointestinal motility and acid secretion (Nillni and Severino, 1999). The biological functions of TRH are mediated by binding to two Gq-coupled seven transmembrane receptors, TRH1 and TRH2 (Sun et al., 2003). TRH1 is present in humans, rodents, and other vertebrates, whereas TRH2 has been identified only in rodents. Synthetic TRH (protirelin) is used clinically to evaluate the functionality of the hypothalamic-pituitary-thyroid axis. The cloned human TRH-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant TRH expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between TRH and its ligands.

    Additional Resource :  HTS126C060515 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 mL per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human TRH cDNA (Accession Number: NM_003301- see CODING SEQUENCE below) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Nillni EA and Sevarino KA (1999) The biology of pro-thyrotropin-releasing hormone-derived peptides. Endocr. Rev. 20: 599-64.
    Reference 2: 2. Sun Y et al. (2003) Thyrotropin-releasing hormone receptors -- similarities and differences. J. Mol. Endocrinol. 30: 87-97.