HTS093L - ChemiBrite™ Human FP Prostanoid Receptor Calcium-Optimized Stable Cell Line

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HTS093L
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    Prostanoids are a series of arachidonic acid metabolites produced by the action of cyclooxygenase and subsequently by isomerases and synthases. Cells rapidly secrete prostanoids after synthesis, whereupon the prostanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The prostaglandin PGF2a binds specifically to the FP receptor, which couples to Gq/11 to mobilize intracellular calcium. Binding of PGF2a to FP receptors in the corpus luteum is required for luteolysis and subsequent parturition in mice (Sugimoto et al., 1998). PGF2a also decreases intraocular pressure by an FP-dependent mechanism, and an PGF2a analog, latanoprost, is used clinically in the treatment of glaucoma (Crowston et al., 2004). FP also contributes to tachycardia induced by inflammatory stimuli (Takayama et al., 2005). 

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    Contents: 2 vials of mycoplasma-free cells, 1 mL per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human FP cDNA (Accession Number: NM_000959- see CODING SEQUENCE below) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Crowston JG et al. (2004) Effect of latanoprost on intraocular pressure in mice lacking the prostaglandin FP receptor. Invest. Ophthalmol. Vis. Sci. 45: 3555-9.
    Reference 2: 2. Narumiya S and FitzGerald GA (2001) Genetic and pharmacological analysis of prostanoid receptor function. J. Clin. Invest. 108: 25-30.
    Reference 3: 3. Sugimoto Y et al. (1997) Failure of parturition in mice lacking the prostaglandin F receptor. Science 277: 681-684.
    Reference 4: 4. Takayama K et al. (2005) Thromboxane A2 and prostaglandin F2a mediate inflammatory tachycardia. Nat. Med. 11: 562-566.
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