HTS079C - ChemiScreen™ Human VPAC2 Receptor Calcium-Optimized Stable Cell Line

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HTS079C
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    Vasoactive intestinal peptide (VIP), a 28 amino acid peptide originally isolated by its vasodilation activity, binds to two class B GPCRs, VPAC1 and VPAC2, to exert its functions in the CNS, vasculature, immune system and adrenal medulla (Harmar et al., 1998). In the immune system, VPAC2 is expressed on stimulated CD4 T cells, and binding of T cell-derived VIP to VPAC2 induces a shift toward the Th2 pathway (Delgado et al., 2004; Voice et al., 2004). In addition, VPAC2 is an essential regulator of the circadian pacemaker of the hypothalamic suprachiasmatic nuclei (Hughes et al., 2004). The cloned human VPAC2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant VPAC2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between VPAC2 and its ligands.

    Additional Resource :  HTS079C060815 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 mL per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human VPAC2 cDNA (Accession Number: NM_003382- see CODING SEQUENCE below) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Delgado M et al. (2004) The significance of vasoactive intestinal peptide in immunomodulation. Pharmacol. Rev. 56: 249-290.
    Reference 2: 2. Harmar AJ et al. (1998) International Union of Pharmacology. XVIII. Nomenclature of receptors for vasoactive intestinal peptide and pituitary adenylate cyclase-activating polypeptide. Pharmacol. Rev. 50: 265-270.
    Reference 3: 3. Hughes AT et al. (2004) Aberrant gating of photic input to the suprachiasmatic circadian pacemaker of mice lacking the VPAC2 receptor. J. Neurosci. 24: 3522-6.
    Reference 4: 4. Voice J et al. (2004) c-Maf and JunB mediation of Th2 differentiation induced by the type 2 G Protein-Coupled Receptor (VPAC2) for vasoactive intestinal peptide. J. Immunol. 172: 7289-7296.
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