HTS056RTA - Ready-to-Assay™ FPRL1 N-formylpeptide Receptor Frozen Cells

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    FPRL1 (formyl peptide receptor-like 1, also known as FPR2) is a GPCR that belongs to the N-formyl peptide receptor family.  Initially described as a receptor for lipoxin A4, FPRL1 has been shown to bind to a synthetic peptide WKYMVm, amyloid beta peptides, and N-formylated mitochondrial peptides and mediates phagocyte chemotaxis (Fiore et al., 1994; Le et al., 1999; Rabiet et al., 2005; Iribarren et al., 2005 Eurofins’ cloned human FPRL1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant FPRL1  expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.  Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at FPRL1.

    Additional Resource :  HTS056RTA092714 Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2. Media Component at 4ºC (-20ºC for prolonged storage).
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exongenous Gene Expression: FPR2 cDNA (Accession Number: NM_001462- see CODING SEQUENCE below) expressed from a proprietary pHS plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Fiore S et al. (1994) Identification of a human cDNA encoding a functional high affinity lipoxin A receptor. J. Exp. Med. 180: 253-260.
    Reference 2: 2. Iribarren P et al. (2005) Role of formyl peptide receptor-like 1 (FPRL1/FPR2) in mononuclear phagocyte responses in Alzheimer disease. Immunol. Res. 31: 165-76.
    Reference 3: 3. Le Y et al. (1999) Utilization of two seven-transmembrane, G protein-coupled receptors, formyl peptide receptor-like 1 and formyl peptide recptor, byt the synthetic hexapeptide WKYMVm for human phagocyte activation. J. Immunol. 163: 6777-6784.
    Reference 4: 4. Rabiet MJ et al. (2005) Human mitochondria-derived N-formylated peptides are novel agonists equally active on FPR and FPRL1, while Listeria monocytogenes-derived peptides preferentially activate FPR. Eur. J. Immunol. 35: 2486-95.