Calcium Flux Assay

HTS056C - ChemiScreen™ Human FPRL1 N-Formylpeptide Receptor Calcium-Optimized Stable Cell Line

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    FPRL1 (formyl peptide receptor-like 1, also known as FPR2) is a GPCR that belongs to the N-formyl peptide receptor family.  Initially described as a receptor for lipoxin A4, FPRL1 has been shown to bind to a synthetic peptide WKYMVm, amyloid beta peptides, and N-formylated mitochondrial peptides and mediates phagocyte chemotaxis (Fiore et al., 1994; Le et al., 1999; Rabiet et al., 2005; Iribarren et al., 2005).  The cloned human FPRL1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant FPRL1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to enhance coupling of the receptor to the calcium signaling pathway.  Thus, the cell line is an ideal tool for screening for antagonists of interactions between FPRL1 and its ligands.

    Additional Resource :  HTS056C050815 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human FPRL1 cDNA (Accession Number: NM_001462) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Fiore S et al. (1994) Identification of a human cDNA encoding a functional high affinity lipoxin A receptor. J. Exp. Med. 180: 253-260.
    Reference 2: 2. Iribarren P et al. (2005) Role of formyl peptide receptor-like 1 (FPRL1/FPR2) in mononuclear phagocyte responses in Alzheimer disease. Immunol. Res. 31: 165-76.
    Reference 3: 3. Le Y et al. (1999) Utilization of two seven-transmembrane, G protein-coupled receptors, formyl peptide receptor-like 1 and formyl peptide recptor, byt the synthetic hexapeptide WKYMVm for human phagocyte activation. J. Immunol. 163: 6777-6784.
    Reference 4: 4. Rabiet MJ et al. (2005) Human mitochondria-derived N-formylated peptides are novel agonists equally active on FPR and FPRL1, while Listeria monocytogenes-derived peptides preferentially activate FPR. Eur. J. Immunol. 35: 2486-95.