HTS055RTA - Ready-to-Assay™ CXCR5 Chemokine Family Receptor Frozen Cells

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HTS055RTA
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    CXCR5 (also known as BLR1) is a GPCR that binds selectively to the chemokine CXCL13 (also known as BLC and BCA-1) to mediate immune system development and function (Förster et al., 1996; Gunn et al., 1998).  CXCR5 cooperates with CCR7 to determine functional organization of lymphoid organs such as lymph nodes and Peyer’s patches (Ohl et al., 2003).  In addition, CXCR5 mediates migration of B cells into the follicles of the splenic white pulp, thus permitting antigen deposition on follicular dendritic cells (Förster et al., 1996;  Cinamon et al., 2008).  Patients with rheumatoid arthritis (RA) displayed upregulated expression of CXCR5 to a greater extent than other chemokine receptors, and CXCR5-null mice subjected to antigen-induced arthritis display reduced joint destruction (Schmutz et al, 2004;  Wengner et al, 2007).  Therefore, CXCR5 represents a potential target for treatment of rheumatoid arthritis. Cloned human CXCR5 -expressing cell line is made in the Chem-10 host, which supports high levels of recombinant CXCR5 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CXCR5.

     

    Additional Resource :  HTS055RTA092714 Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2. Media Component at 4ºC (-20ºC for prolonged storage).
    Applications: Calcium Flux Assay
    Host Cell: Chem-10, an adherent rat hematopoietic cell line expressing endogenous G·15 protein as well as an exogenous proprietary promiscuous Gα protein.
    Exongenous Gene Expression: CXCR5 cDNA (Accession Number: NM_001716- see CODING SEQUENCE below) expressed from a proprietary pHS plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Cinamon G et al. (2008) Follicular shuttling of marginal zone B cells facilitates antigen transport. Nat. Immunol. 9: 54-62.
    Reference 2: 2. Gunn MD et al. (1998) A B-cell-homing chemokine made in lymphoid follicles activates Burkitt’s lymphoma receptor-1. Nature 391: 799-803.
    Reference 3: 3. Ohl L et al. (2003) Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs. J. Exp. Med. 197: 1199-1204.
    Reference 4: 4. Schmutz C et al. (2004) Chemokine receptors in the rheumatoid synovium: upregulation of CXCR5. Arthritis Res. Ther. 7: R217-R229.
    Reference 5: 5. Wengner AM et al. (2007) CXCR5- and CCR7-dependent lymphoid neogenesis in a murine model of chronic antigen-induced arthritis. Arthritis Rheum. 56: 3271-3283.
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