HTS055C - ChemiSCREEN™ Human Recombinant CXCR5 Chemokine Receptor Calcium-Optimized Stable Cell Line

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    CXCR5 (also known as BLR1) is a GPCR that binds selectively to the chemokine CXCL13 (also known as BLC and BCA-1) to mediate immune system development and function (Förster et al., 1996;  Gunn et al., 1998).  CXCR5 cooperates with CCR7 to determine functional organization of lymphoid organs such as lymph nodes and Peyer’s patches (Ohl et al., 2003).  In addition, CXCR5 mediates migration of B cells into the follicles of the splenic white pulp, thus permitting antigen deposition on follicular dendritic cells (Förster et al., 1996;  Cinamon et al., 2008).  Patients with rheumatoid arthritis (RA) displayed upregulated expression of CXCR5 to a greater extent than other chemokine receptors, and CXCR5-null mice subjected to antigen-induced arthritis display reduced joint destruction (Schmutz et al, 2004;  Wengner et al, 2007).  Therefore, CXCR5 represents a potential target for treatment of rheumatoid arthritis.  Cloned human CXCR5-expressing cell line is made in the Chem-10 host, which supports high levels of recombinant CXCR5 expression on the cell surface and contains optimal levels of promiscuous G protein to couple the receptor to the calcium signaling pathway.  Thus, the cell line is an ideal tool for screening for agonists and antagonists at CXCR5.

    Additional Resource :  HTS055C032315 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-10, a rat adherent cell line of hematopoietic origin expressing a recombinant promiscuous G-protein.
    Exongenous Gene Expression: Human CXCR5 cDNA (Accession Number: NM_0001716- see CODING SEQUENCE below)
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Cinamon G et al. (2008) Follicular shuttling of marginal zone B cells facilitates antigen transport. Nat. Immunol. 9: 54-62.
    Reference 2: 2. Gunn MD et al. (1998) A B-cell-homing chemokine made in lymphoid follicles activates Burkitt’s lymphoma receptor-1. Nature 391: 799-803.
    Reference 3: 3. Ohl L et al. (2003) Cooperating mechanisms of CXCR5 and CCR7 in development and organization of secondary lymphoid organs. J. Exp. Med. 197: 1199-1204
    Reference 4: 4. Schmutz C et al. (2004) Chemokine receptors in the rheumatoid synovium: upregulation of CXCR5. Arthritis Res. Ther. 7: R217-R229
    Reference 5: 5. Wengner AM et al. (2007) CXCR5- and CCR7-dependent lymphoid neogenesis in a murine model of chronic antigen-induced arthritis. Arthritis Rheum. 56: 3271-3283.