HTS054RTA - Ready-to-Assay™ CXCR6 Chemokine Family Receptor Frozen Cells

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    The GPCR CXCR6 (previously known as BONZO, STRL33 and TYMSTR) binds selectively to the free chemokine domain of CXCL16, which is derived from a membrane-bound precursor containing a CXC-containing chemokine domain, a glycosylated mucin-like domain, and a transmembrane domain (Wilbanks et al., 2001).  CXCR6 is selectively expressed on Th1, Th2 and Tr1 T cell subsets, whereas CXCL16 is expressed on monocytes/macrophages and dendritic cells (Tabata et al., 2005).  CXCR6 functions as a cofactor with CD4 for HIV entry and Env-mediated cell fusion (Liao et al., 1997).  Binding of CXCL16 to CXCR6 promotes migration of activated lymphocytes to sites of inflammation in tissues such as liver and synovium (Nanki et al., 2005, Sato et al., 2005). Millipore’s cloned human CXCR6 -expressing cell line is made in the Chem-5 host, which supports high levels of recombinant CXCR6 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at CXCR6.

    Additional Resource :  HTS054RTA092714 Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2. Media Component at 4ºC (-20ºC for prolonged storage).
    Applications: Calcium Flux Assay
    Host Cell: Chem-5, an adherent rat hematopoietic cell line expressing endogenous G·15 protein as well as an exogenous proprietary promiscuous Gα protein.
    Exongenous Gene Expression: CXCR6 cDNA (Accession Number: NM_006564- see CODING SEQUENCE below) expressed from a proprietary pHS plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Liao F et al. (1997) STRL33, A novel chemokine receptor-like protein, functions as a fusion cofactor for both macrophage-tropic and T cell line-tropic HIV-1. J. Exp. Med. 185: 2015-23.
    Reference 2: 2. Nanki T et al. (2005) Pathogenic role of the CXCL16-CXCR6 pathway in rheumatoid arthritis. Arthritis Rheum. 52: 3004-3014.
    Reference 3: 3. Sato T et al. (2005) Role for CXCR6 in recruitment of activated CD8+ lymphocytes to inflamed liver. J. Immunol. 174: 277-283.
    Reference 4: 4. Tabata S et al. (2005) Distribution and kinetics of SR-PSOX/CXCL16 and CXCR6 expression on human dendritic cell subsets and CD4+ T cells. J. Leukoc. Biol. 77: 777-786.
    Reference 5: 5. Wilbanks A et al. (2001) Expression cloning of the STRL33/BONZO/TYMSTR ligand reveals elements of CC, CXC, and CX3C chemokines. J. Immunol. 166: 5145-5154