Calcium Flux Assay

HTS054C - ChemiSCREEN™ Human Recombinant CXCR6 Chemokine Receptor Calcium-Optimized Stable Cell Line

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    The GPCR CXCR6 (previously known as BONZO, STRL33 and TYMSTR) binds selectively to the free chemokine domain of CXCL16, which is derived from a membrane-bound precursor containing a CXC-containing chemokine domain, a glycosylated mucin-like domain, and a transmembrane domain (Wilbanks et al., 2001).  CXCR6 is selectively expressed on Th1, Th2 and Tr1 T cell subsets, whereas CXCL16 is expressed on monocytes/macrophages and dendritic cells (Tabata et al., 2005).  CXCR6 functions as a cofactor with CD4 for HIV entry and Env-mediated cell fusion (Liao et al., 1997).  Binding of CXCL16 to CXCR6 promotes migration of activated lymphocytes to sites of inflammation in tissues such as liver and synovium (Nanki et al., 2005, Sato et al., 2005).  The cloned human CXCR6 -expressing cell line is made in the Chem-5 host, which supports high levels of recombinant CXCR6 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway.  Thus, the cell line is an ideal tool for screening for agonists and antagonists of CXCR6.  

    Additional Resource :  HTS054C050715 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-5, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human CXCR6 cDNA (Accession Number: NM_006564- see CODING SEQUENCE below) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Liao F et al. (1997) STRL33, A novel chemokine receptor-like protein, functions as a fusion cofactor for both macrophage-tropic and T cell line-tropic HIV-1. J. Exp. Med. 185: 2015-23.
    Reference 2: 2. Nanki T et al. (2005) Pathogenic role of the CXCL16-CXCR6 pathway in rheumatoid arthritis. Arthritis Rheum. 52: 3004-3014.
    Reference 3: 3. Sato T et al. (2005) Role for CXCR6 in recruitment of activated CD8+ lymphocytes to inflamed liver. J. Immunol. 174: 277-283.
    Reference 4: 4. Tabata S et al. (2005) Distribution and kinetics of SR-PSOX/CXCL16 and CXCR6 expression on human dendritic cell subsets and CD4+ T cells. J. Leukoc. Biol. 77: 777-786.
    Reference 5: 5. Wilbanks A et al. (2001) Expression cloning of the STRL33/BONZO/TYMSTR ligand reveals elements of CC, CXC, and CX3C chemokines. J. Immunol. 166: 5145-5154.