HTS053RTA - Ready-to-Assay™ XCR1 Chemokine Family Receptor Frozen Cells

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    The migration of leukocytes from the bloodstream to sites of inflammation is a dynamic factor involving adhesion molecules and chemotactic factors. Chemokines play a role in the trafficking of leukocytes by inducing cellular motility and activating adhesion molecules within the immune system.  Lymphotactin (also known as XCL1 and SCM-1) is a unique chemokine that retains only two of the four cysteine residues found in the CC, CXC and CX3C families of chemokines.  A Gi-coupled receptor, XCR1, binds to lymphotactin and mediates its chemotactic effects (Yoshida et al., 1998).  Chemokines promote accumulation of activated mononuclear cells (MNCs) in inflamed joints in rheumatoid arthritis (RA) and lymphotacin is highly expressed in synovial fluid of RA patients. In situ hybridization studies indicate that XCR1 expression was detected in both the infiltrated MNCs and the synoviocytes from synovial specimens taken from RA patients (Wang et al., 2004). Cloned human XCR1-expressing cell line is made in the Chem-4 host, which supports high levels of recombinant XCR1 expression on the cell surface and contains optimized levels of a recombinant promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at XCR1.

    Additional Resource :  HTS053RTA092414 Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2. Media Component at 4ºC (-20ºC for prolonged storage).
    Applications: Calcium Flux Assay
    Host Cell: Chem-4, an adherent rat hematopoietic cell line expressing endogenous G·15 protein as well as an exogenous proprietary promiscuous Gα protein
    Exongenous Gene Expression: XCR1 cDNA (Accession Number: NM_005283.- see CODING SEQUENCE below) expressed from a proprietary pHS plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Wang CR et al. (2004) Up-regulation of XCR1 expression in rheumatoid joints. Rheumatology: 43: 569-73.
    Reference 2: 2. Yoshida T et al. (1998) Identification of single C motif-1/lymphotactin receptor XCR1. J. Biol. Chem. 273: 16551-16554.