Calcium Flux Assay

HTS053C - ChemiSCREEN™ Human Recombinant XCR1 Chemokine Receptor Calcium-Optimized Stable Cell Line

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    The migration of leukocytes from the bloodstream to sites of inflammation is a dynamic factor involving adhesion molecules and chemotactic factors. Chemokines play a role in the trafficking of leukocytes by inducing cellular motility and activating adhesion molecules within the immune system.  Lymphotactin (also known as XCL1 and SCM-1) is a unique chemokine that retains only two of the four cysteine residues found in the CC, CXC and CX3C families of chemokines.  A Gi-coupled receptor, XCR1, binds to lymphotactin and mediates its chemotactic effects (Yoshida et al., 1998).  Chemokines promote accumulation of activated mononuclear cells (MNCs) in inflamed joints in rheumatoid arthritis (RA) and lymphotacin is highly expressed in synovial fluid of RA patients. In situ hybridization studies indicate that XCR1 expression was detected in both the infiltrated MNCs and the synoviocytes from synovial specimens taken from RA patients (Wang et al., 2004).  The cloned human XCR1-expressing cell line is made in the Chem-4 host, which supports high levels of recombinant XCR1 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway.  Thus, the cell line is an ideal tool for screening for antagonists of interactions between XCR1 and its ligands.

    Additional Resource :  HTS053C060815 Datasheet

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    Contents: 2 vials of mycoplasma-free cells, 1 ml per vial.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Host Cell: Chem-4, an adherent cell line expressing the promiscuous G-protein, Gα15.
    Exongenous Gene Expression: Human XCR1 cDNA (Accession Number: NM_005283- see CODING SEQUENCE below) and promiscuous G protein are expressed in a bicistronic vector
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Wang CR et al. (2004) Up-regulation of XCR1 expression in rheumatoid joints. Rheumatology: 43: 569-73.
    Reference 2: 2. Yoshida T et al. (1998) Identification of single C motif-1/lymphotactin receptor XCR1. J. Biol. Chem. 273: 16551-16554.