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HTS045RTA - Ready-to-Assay™ OX2 Orexin Receptor Frozen Cells

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    The orexin/hypocretin peptides orexin A and orexin B (also known as hcrt-1 and hcrt-2) are 33- and 28-amino acid peptides, respectively, that are preferentially expressed in hypothalamus.  The orexins bind to two GPCRs, OX1 and OX2, to increase intracellular calcium.  Orexins function to increase wakefulness, primarily through OX2, which has been shown to harbor the causative mutation in narcoleptic dogs (Lin et al., 1999).  In addition, the orexinergic system plays a role in feeding and stress responses (Kukkonen et al., 2002). Cloned human OX2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant OX2 expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at OX2.

    Additional Resource: HTS045RTA091914_Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2.
    Applications: Calcium Flux Assay
    Entrez Gene Number: NM_001526
    Protein Targets: OX2
    Target Sub-family: Orexin
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exogenous Gene Expression: HCRTR2 cDNA (Accession Number: NM_001526) expressed from a proprietary promoter plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Kukkonen JP et al. (2002) Functions of the orexinergic/hypocretinergic system. Am. J. Physiol. Cell Physiol. 283: C1567-C1591.
    Reference 2: 2. Lin L et al. (1999) The sleep disorder canine narcolepsy is caused by a mutation in the hypocretin (orexin) receptor 2 gene. Cell .98: 365-376.