HTS040RTA - Ready-to-Assay™ NOP1 Opioid Receptor Frozen Cells

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    The NOP1 receptor (also known as ORL1) is related to the opioid receptor family of GPCRs but does not bind to classical opioids.  An endogenous ligand for NOP1 has been characterized and termed orphanin FQ or nociceptin (OFQ/N), which in turn does not bind to other members of the opioid receptor family.  NOP1 is expressed widely in the CNS, and binding of OFQ/N to NOP1 appears to function in nociception, locomotor activity, anxiety, reward, memory and tolerance to classical opioids (Mogil and Pasternak, 2001). Cloned human NOP1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant NOP1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at NOP1.

    Additional Resource :  HTS040RTA092714 Datasheet

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    Item Unit of Measure: PK
    Contents: Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
    Storage: Vials are to be stored in liquid N2. Media Component at 4ºC (-20ºC for prolonged storage).
    Applications: Calcium Flux Assay
    Host Cell: Chem-1, an adherent rat hematopoietic cell line expressing endogenous Gα15 protein.
    Exongenous Gene Exoression: ORL1 cDNA (Accession Number: NM_000913- see CODING SEQUENCE below) expressed from a proprietary pHS plasmid.
    GMO: This product contains genetically modified organisms.
    Reference 1: 1. Mogil J.S. and Pasternak G.W. (2001) The molecular and behavioral pharmacology of the orphanin FQ/nociceptin peptide and receptor family. Pharmacol. Rev. 53: 381-415.