HTS039M - ChemiSCREEN™ D2 Dopamine Receptor Membrane Preparation

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    Dopamine is a catecholamine neurotransmitter that functions in the CNS to control locomotor, cognitive, emotional and neurendocrine processes, and in the periphery to modulate cardiovascular, renal and gastrointestinal processes.  The biological activities of dopamine are mediated by a family of five GPCRs.  The D1 and D5 subtypes couple to Gs to increase intracellular cAMP, whereas the D2, D3 and D4 subtypes couple to Gi to reduce cAMP (Missale et al., 1998). The D2 dopamine receptors have been of particular clinical interest due to their regulation of prolactin secretion and their affinity for antipsychotic drugs.  The D2 receptor exists as two alternatively spliced isoforms differing in the insertion of a stretch of 29 amino acids in the third intracellular loop (D2S and D2L) (Giros et al., 1989; Grandy et al., 1989).  D2L dopamine receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening for agonists and antagonists of the D2 dopamine receptor.  

    Additional Resource :  HTS039M090414 Datasheet

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    Item Unit of Measure: EA
    Quantity: 200 units
    Storage: On receipt of material store at -70°C. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles.
    Applications: GTPγS Binding
    Species: Human DRD2 encoding D2 Dopamine Receptor long isoform (D2L- Accession number NM_000795)
    Host Cell: Chem-1, an adherent mammalian cell line without any endogenous D2 Dopamine Receptor expression.
    Reference 1: 1. Grandy DK et al. (1989) Cloning of the cDNA and gene for a human D2 dopamine receptor. Proc. Natl. Acad. Sci. U S A. 86: 9762-6.
    Reference 2: 2. Giros B et al. (1989) Alternative splicing directs the expression of two D2 dopamine receptor isoforms. Nature 342: 923-6
    Reference 3: 3. Missale C et al. (1998) Dopamine receptors: from structure to function. Physiol. Rev. 78: 189-225.